Chen Rong Huei; Wei Yu Chen; Shang Ta Wang; Chu Hsi Hsu; Min Lang Tsai (2009). The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear -(1-6)-linked glucose chain with however a low percentage (2-5%) of -(1-3) branches distributed along the chain4. 1905/05) for their financial support. Kasaai, Mohammad R. (2012). Biomacromolecules 8, 2464-2475. Keywords: WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Proc. Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861. Molecular weight, molecular weight distribution and molecular size of a native dextran. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. This conjecture found to be correct. The M-H equation is empirical and valid for monodisperse polymers, and it is applied to biomacromolecules (complex, hyperbranched, crosslink), taking the precaution of comparing the value of Mv with the polydispersity index (qM-H). Prausnitz MR, Langer R. Transdermal drug delivery. Gold MH, Biron JA. In addition, we evaluated the regeneration of SC after microporation. (1999). Unauthorized use of these marks is strictly prohibited. J. Phys. The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. Dilute solution properties and degree of chain branching for dextran. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. Fluorescent and Biotinylated DextransSection 14.5 Terms and Conditions, FITC-dextran solution (600L) was added to the donor chamber. Charge: Anionic. Enhanced topical delivery of small hydrophilic or lipophilic active agents and epidermal growth factor by fractional radiofrequency microporation. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. Karmarkar, S.; Garber, R.: Kluza, J.; Koberda, M. (2006). WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. De Belder, A. N. (2001). ; Rani, K.Y. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. AATEX. Polymolecularity and polydispersity in Molecular weight determinations. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Stokes radius The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. Determination of constants, kM-H and a, from the intrinsic viscosity data, requires a series of monodisperse polymer samples with known molecular weight or a series of polydisperse polymer samples with known viscosity average molecular weights, Mv. (2013). There is a deviation from linearity after 580kDa [69, 70]. Structural and biocompatibility properties of dextran from Radiofrequency ablation of malignant liver tumors. Gillespie, T.; Hulme, M. A. (2006). Beer, M.U. The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. If there are particles in the sample, the incident laser light gets scattered in all directions. 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. Curvale, R.; Masuelli, M.; Perez Padilla, A. Hydrodynamic radii were measured using Dynamic Light Scattering (DLS) in a Malvern Zetasizer NanoZS DLS (Malvern Instruments, Malvern, UK) at high scattering angle (173) to minimise the effects of dust and supramolecular contamination (NIBS, 173). Nordmeier, Eckliard (1993). Ann Surg Oncol. Soc., 16, 3A, 426-433. Ortega, A.; Garca de la Torre, J. The reason for a nonlinear Mark-Houwink relationship is confirmed by the intrinsic viscosity which in contrast with scaling prediction shows no power law behavior. These values indicate for dextrans in dilute aqueous solution, both methods give very similar results of the intrinsic viscosity and the respective Mw. To confirm the extent of skin damage and regeneration after microporation, porations were performed 2, 5, and 10 times. 8. Differences were considered statistically significant at p <0.05. Pharmaceut Sci Tech. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. B 113, 8020-8025. WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. Characterisation of insulin analogues therapeutically available to Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Fixable: Fixable via Lysine. In a good solvent, a temperature increase results in an intrinsic viscosity decrease and in a less-extended conformation (RH<), because the entropy value increases with an increase in temperature and it is unfavorable for an extended conformation. ; Tsuchiya, H.M.; Rist, C.E. Principles of Size Exclusion Chromatography SEC (GFC 2012;3(1):208. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. Ind. Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. ; Picout, D.R. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. Chem. Trop J Pharm Res. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. Carbohydrate Polymers 88 373-381. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Biomaterials Research The hydrodynamic 57 radius of the dextranase is 7 For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. 2018;1:10915. ; Patel, T.R. The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. Thus, it is accepted that, for every flexible-chain polymer in a (ideal) solvent, there is a universal value of 0 = 2.1-2.5x1023mol-1 [52]. Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. 2012;101(8):28619. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. Dilute Solution. Dextran 670 GPC Standard | Pharmacosmos Dextran The mechanism of the dextran-induced red blood cell Aggregation. Ghimici, Luminita; Nichifora, Marieta; Eich, Andreas; Wolf, Bernhard A. Recent Pat Drug Deliv Formul. Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time. Int J Pharm Investig. Monolayers and Thin Films of Dextran Hydrophobically Modified. Human cadaver skin has been confirmed as alive skin tissue through analysis of its morphology and enzymes, and its usefulness as an alternative skin membrane in drug permeation experiments has been verified [20]. Journal of Polymer Science Part B: Polymer Physics, 38, 2591-2598. Pore closure examination was performed using an optical microscope (BX43, Olympus, Japan) immediately (time 0), 4, 8, and 24h following device application. Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. These results are in agreement with a previous study which suggested that the microporation using radiofrequency causes disruption of the SC, enabling the percutaneous permeation of the FITC-dextran [21]. volume24, Articlenumber:22 (2020) Journal of Chemical Education, 49(8), 577-579. Macromolecules. Theory, Estimation, Experiment, and Data. In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation. 2003;10:33847. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). After 24h, the cumulative permeation of FITC-dextran M.W. 56 The linkage that the enzyme dextranase breaks down is indicated by the black arrow. Epub 2022 Feb 2. Journal Argentine Chemical Society 100 (2013) 25-34. Carbohydrate Polymers, 79, 380-390. Careers. We observe that this maximum radius is below Vemulapalli V, Bai Y, Kalluri H, Herwadkar A, Kim H, Davis SP, Friden PM, Banga AK. The range of temperatures used was 25-50 (0.1) C and it was regulated by thermostatic bath Haake C. The viscosity measurements required Hagenbach-Couette correction time (tg 37.31s). Solution properties of branched dextran. Amersham Biosciences, AA Edition, www.amershambiosciences.com. (1987). dextran Hydrodynamic (2013). dextran hydrodynamic radius Hydrodynamic Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. Various average molecular weights (Mv, Mn, Mw, and Mz) instead of Mv were substituted in M-H equation. For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ]. (2003). The formula is verified for different systems of polymer-solvent and the values are in accord with those obtained by extrapolation. 2004;25(9):155967. dextran Table 5 shows the data of Mark-Houwink parameters determined in figure 4 from linear regressions with 2 values higher than 0.9797. Advances in chemistry series Washington, DC: American Chemical Society., pp. 2000;3(9):31826. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. Carbohydrate Polymers, 68, 477-488. IV. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Chen Rong Huei; Min Lang Tsai (1998). (1954). Weissella: An Emerging Bacterium with Promising Health Benefits. J. Braz. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. (2012). Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection 2009;6(4):34354. Table 2 compares the properties of nanoparticles coated with one or other polymer. dextran hydrodynamic radius. Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility. (2008). Food Funct. ; Bataille, B. hydrodynamic Bohdanecky, Miloslav (1996). Viswanath, D.S. When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. Biotechnology Progress, 19, 544-548. Anal. (2000). WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Figure5 shows the cumulative amount of the FITC-dextran (M.W. WebDextran 40 Pharmaceutical Quality. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). Ibrahim O, Munavalli GS, Dover JS. Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. 2. Ficoll and dextran vs. globular proteins as probes for testing Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. 2012;87(6):1728. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was dextran The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. Solutions of dextrans and blue dextrans (0.21.0 g/L) were prepared in 0.200 M NaCl (aq). Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Critical parameters for gel permeation High Manufacturing Standards of Molecular Probes Dextrans. The Mark-Houwink parameters and the effect of temperature on them are specifically studied. (1999). Harding, Stephen E. (2005). Biomaterials. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. These hydrodynamic parameters show that these polysaccharides behave as a compact rigid sphere and contract by the increase of temperature (RH decreases) for the Mw range from 8.8 to 200kDa. J Cosmet Laser Ther. Because of the recent animal protection rules which have restricted animal experiments [19], we used human cadaver skin as an alternative to animals. 2013;3(5):3588605. Probiotics Antimicrob Proteins. The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. Walkenhorst, S.; Olivier, R. (2002). Gums and stabilisers for the Food Industry, 5, 447-450. (2008). Biophysical Journal, 27, 177-181. RBECs were passaged onto cell culture inserts as described and cultured for a further 714 days in vitro. Journal of American Chemical Society, 64(11), 2716-2718. These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. The .gov means its official. Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. 2008;26(11):12618. The Hydrodynamic Radii of Macromolecules and Their (2001). @ Nilimesh See attached. We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. 2012;29(7):201729. Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). Journal Non-Newtonian Fluid Mech., 97, 295-301. 2009;8(2):1739. dextran hydrodynamic radius Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.".
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